黄羽肉鸡群体遗传变异的微卫星分析及其与体重杂种优势的关系

选用10对微卫星引物分析了黄羽肉鸡A、B、C3个品系的遗传结构和差异,并对微卫星标记所计算的个体间遗传距离与C×B、C×A杂交后代的初生至8周龄体重的杂种优势和杂种优势率进行了相关分析。结果表明,10个微卫星标记中有9个表现出多态性,多态信息含量(PIC)和群体杂合度(Heterozigosity)分别为0·4400和0·6811,各品系、各微卫星基因座上都表现出一定差异。利用9个多态微卫星标记计算得出的亲本个体间的遗传距离与后代杂种优势率间多数没有显著的相关(P>0·05),仅C×B组合中亲本个体间平均遗传距离与第8周龄体重杂种优势和杂种优势率间相关显著(P<0·05)。     

Habitat Selection and Breeding Success in a Forest-nesting Alcid, the Marbled Murrelet, in Two Landscapes with Different Degrees of Forest Fragmentation

We studied habitat selection and breeding success in marked populations of a protected seabird (family Alcidae), the marbled murrelet (Brachyramphus marmoratus), in a relatively intact and a heavily logged old-growth forest landscape in south-western Canada. Murrelets used old-growth fragments either proportionately to their size frequency distribution (intact) or they tended to nest in disproportionately smaller fragments (logged). Multiple regression modelling showed that murrelet distribution could be explained by proximity of nests to landscape features producing biotic and abiotic edge effects. Streams, steeper slopes and lower elevations were selected in both landscapes, probably due to good nesting habitat conditions and easier access to nest sites. In the logged landscape, the murrelets nested closer to recent clearcuts than would be expected. Proximity to the ocean was favoured in the intact area. The models of habitat selection had satisfactory discriminatory ability in both landscapes. Breeding success (probability of nest survival to the middle of the chick rearing period), inferred from nest attendance patterns by radio-tagged parents, was modelled in the logged landscape. Survivorship was greater in areas with recent clearcuts and lower in areas with much regrowth, i.e. it was positively correlated with recent habitat fragmentation. We conclude that marbled murrelets can successfully breed in old-growth forests fragmented by logging.     

香蕉A基因组品种间遗传关系的SSR检测

应用SSR技术,对32个香蕉A基因组类型品种(系)的遗传关系进行了检测。40对SSR引物在32个品种(系)中分别扩增带数在3～15个,平均每个SSR座位可检测2.99个多态性带;引物的多态信息量(PIC)在0.00～0.88,平均0.62。依据SSR数据计算的品种间遗传距离在0.00%～34.27%,平均12.45%,大多数品种间的遗传变异非常有限,但也存在着遗传差异突出的品种:FHIA25、Yangambi KM5、Pisang Jari Buaya、Rose和皇帝蕉。依据26%的遗传距离,除了FHIA25和Pisang Jari Buaya单独化成1组外,其它30个品种可以分为2组:品种间遗传差异相对较高的组I和品种间遗传差异相对较低的组Ⅱ。Williams与引进的洪都拉斯3号、M931之间,洪都拉斯1号和洪都拉斯2号之间,高脚青芽蕉和高脚顿地雷分别没有区分开来,这可能是同物异名,也可能是同一品种未能分辨的突变体。     

AFLP analysis of Russian <Emphasis Type="Italic">Alternaria tenuissima</Emphasis> populations from wheat kernels and other hosts

Alternaria tenuissima is a common pathogen on a number of plants described in several geographic regions of the world. Genetic variation within and between Russian Far East, North West and Caucasus populations of A. tenuissima from wheat was examined. In addition, genetic differences between isolates from various hosts were estimated. In total, 101 isolates of A. tenuissima were studied using amplified fragment length polymorphism (AFLP) with four primer combinations. Wright’s fixation index (F _st), gene flow (N _m) and gene diversity (H _s) were calculated. AFLP banding patterns indicated significant genetic distance and at the same time a low level of gene flow between the Far East and the two other groups of isolates originating from the European part of country. The degree of similarity between the North West and Caucasus populations was very high, as was the migration rate. Isolates analysed by UPGMA-based cluster analysis were grouped according to location of origin but irrespective of plant host. Based on the F _st value, the group of isolates originating from wheat and barley were not found to differ significantly from each other.     

基于DSP与超声波测距的农业机器人定位与避障控制

随着新兴电子集成技术和自动化技术的发展,控制系统已逐渐向数字化转变,高集成芯片广泛应用于自动化控制领域,体积小、运算能力强的嵌入式系统慢慢开始取代计算机。为此,首先分析了超声波测距功能及其优越性,采用三球定位技术,设计研究了一种基于DSP和超声波的全局定位系统;然后,采用多路超声波收发模块设计了基于超声波的自主避障控制系统,并提出一种新的模糊推理方法,实现机器人的避障和路径规划功能;最后,采用Visual C++可视化程序设计软件对避障系统进行仿真和场地试验,验证了系统的可靠性和可行性,为机器人的研究和发展提供了更加宽广的空间。     

AX=b在逆M-阵类中的反问题

将线性方程组 Ａ Ｘ＝ ｂ 分为５ 种情况（ Ｘ＞ ０、 Ｘ≥０、 Ｘ＜ ０、 Ｘ≤０、 Ｘ 为一般情况），通过构造矩阵的方法，讨论了该线性方程组的反问题在逆 Ｍ－ 阵类中有解的条件．在一般情况下，当给定的实向量 Ｘ 与ｂ 中相应的分量同号时，则线性方程组 Ａ Ｘ＝ ｂ 在逆 Ｍ － 阵类中的反问题有解．     

Discrimination of ‘San Marzano’ accessions: A comparison of minisatellite, CAPS and SSR markers in relation to morphological traits

‘San Marzano’ (SM) is one of the most widely known tomato (Solanum lycopersicum L.) cultivars, and is a classic example of a local variety with a premium value. Unfortunately, the original cultivated form is underrepresented in the Protected Denomination of Origin (PDO) area because of the incidence of contaminant and phenotypically similar genotypes. Our aim was to examine the ability of three DNA marker systems (minisatellite, cleaved amplified polymorphic sequence (CAPS) and simple sequence repeat (SSR)) to reveal the genetic diversity of tomato accessions that were, based on a morphological analysis, very similar. The data indicate that both minisatellites and SSRs can be used to genetically distinguish the analysed materials. Furthermore, these two marker systems depict relationships consistent with the hierarchal pattern obtained by the morphological data. As locally cultivated tomato accessions are often characterised by some degree of genetic variability, our results will be valuable in facilitating the purification, management and breeding of tomato germplasms. The differences between the marker systems employed are also discussed in relation to their usefulness in the agro-food chain.     

ISTR在部分柿属植物种质鉴定和亲缘关系分析中的应用

 利用反向序列标签重复技术( inverse sequence-tagged repeat, ISTR) , 对柿属7个种, 包括柿(Diospyros kaki Thunb. ) 、君迁子(D. lotus L. ) 、浙江柿(D. glaucifolia Metc. ) 、油柿(D. oleifera Cheng. ) 、金枣柿(D. sp. ) 、老鸦柿(D. rhombifolia Hemsl. ) 和美洲柿(D. virginiana L. ) 共32个基因型进行了种质鉴定和亲缘关系研究。结果表明: ISTR可区分供试柿属植物中的30份; ISTR能够很好地适用于柿属植物亲缘关系分析; 供试的中国与日本原产柿种质分别聚类; 新发现的中国甜柿变异类型与日本甜柿的亲缘关系较远且存在较丰富的遗传变异, 可能是潜在的育种资源。ISTR标记可在柿属植物种质资源鉴定和亲缘关系分析中更广泛地应用。     

一种基于神经网络的结构参数识别方法

提出了一种基于神经网络的结构参数识别方法，该方法以残余力向量作为结构参数识别的网络输入。针对训练样本在数据空间分布不均匀的问题，采用ＧＳＬ变换对训练样本数据进行预处理。从而提高了网络收敛速度及参数识别精度。本文算例说明了方法的有效性。     

基于神经网络逆控制方法的水轮发电机组非线性控制器仿真研究

基于水轮发电机组刚性水击模型和神经网络逆系统控制方法,在分析励磁与导叶系统可逆性的基础上提出了一种新的水轮发电机组解耦控制器设计方法.理论分析和仿真结果表明该控制策略较好的解决了水轮发电机组控制时存在的多变量、非线性、强耦合问题;实现了系统的动态解耦控制,具有较好的适应性和鲁棒性,因此可有效增强输电系统的暂态稳定性.